ANTIMICROBIAL TEST OF “TUTUP” FLOWERS (Macaranga tanarius (L.) Mull.Arg.)

Abstract

Preservation “siwalan†sap in Lamongan done in two ways, namely by the addition of lime solution
and by addition of liquid of “tutup†flowers. “Tutup†flowers ((Macaranga tanarius (L.) Mull.Arg.) (name
of Lamongan district) is included in the family Euphorbiaceae. Sugar is produced from the sap with liquid
of “tutup†flowers preservative have better quality than the other sap preservative. The results also showed
that the sap with liquid of “tutup†flowers preservative have a longer shelf life, with a fewer the number of
total microbes. Therefore, it needs to be studied a potential “tutup†flowers extract as an antiâ€microbial
material. The purpose of this study is as follows: (1) to know and effectiveness test of “tutup†flowers preservative
against pathogenic microorganisms and food destroyer, (2) to determine part of “tutup†plants
which potentially as a preservative, and (3) to determine the minimum concentration of preservative with
Contact Method.
The results showed the following: (1) Growth of Saccharomyces cerevisiae and Staphylococcus aureus
can be inhibited by liquid of “tutup†flowers, but the growth of Pseudomonas fluorecens are not inhibited
by liquid of “tutup†flowers; (2) Pseudomonas fluorecens bacterial growth can be inhibited by a solution of
lime, but the growth of Staphylococcus aureus and the Saccharomyces cereviseae not inhibited by a solution
of lime; (3) Inhibited the growth of Staphylococcus aureus by addition of “tutup†plant leaves extract
with concentration 30 % and “tutup†plant flowers extract with 20% and 30% concentration, inhibited the
growth of Pseudomonas fluorecens by addition of “tutup†plant leaves and flowers with 20% and 30% concentration,
whereas inhibited the growth of Saccharomyces cereviceae by the addition of leaf extract of
“tutup†plant with a concentration of 20% and 30% and a flower extract of “tutup†plant with a minimum
concentration of 10%; and (4) furthermore used flower extract of “tutup†plant with concentration of at
least 20% can inhibited the growth of Staphylococcus aureus, Pseudomonas fluorecens, and Saccharomyces
cereviceae.
Key words: “tutup†flowers (Macaranga tanarius); antimicrobial; natural preservatives