AVOID CONTAMINATION IN SOYBEAN (Glycine max, L. [Merrill]) MICROSPORES CULTURE

Sumarmi -

Abstract


Microspore culture is done to obtain pure strains. The purpose of soybean microspore culture to obtain
quality seeds. Two important step that must be done is isolation of microspores in starvation medium and
subculture into embryogenesis medium. Many factors contributing to the contamination of soybean
microspore culture. Contamination in the B medium temperature 34 0C is more common than 4 0C. Vulnerable
to contamination because of embryogenesis medium rich in nutrients. Bacterial contamination
can be caused by internal contaminants such as shape of the anther. Other internal contaminants that
cause diseases such as fungi Colletotrichum truncatum and Phakopsora pachyrhizi. Antagonistic fungi
which contaminate cultures that Trichoderma spp., Alternaria spp., Fusarium spp. Handling of contamination
is done by selecting the appropriate methods in order to remain viable microspores. Sterilization soybean
flower buds with 20% Tween for 10 minutes and then rinsed with distilled water. Moreover sterilization
with 4% Hg Cl2 and 10% NaOCl for 10 minutes, rinsed with distilled water times, followed by 96%
alcohol for 1 minute, can press up to 70% contamination.
Keywords: soybean, microspore, contamination